Metastasis, the spread of cancer cells from the primary tumor to distant organs, is the main cause of cancer deaths. Cancer cells invade surrounding tissue by losing cell-cell adhesion, often through epithelial-mesenchymal transition (EMT), and degrading the extracellular matrix with matrix metalloproteinases (MMPs), cathepsins and the urokinase plasminogen activator system. They then enter the blood or lymphatic vessels, survive in the circulation, exit at distant sites and grow into metastases. Genes involved include adhesion molecules, MMPs and TIMPs, chemokines and their receptors, and metastasis suppressors.
A ready-to-use Human Tumor Invasion/Metastasis PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against tumor invasion/metastasis genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: ADAMTS1 ADAM metallopeptidase ALDH3A1 Aldehyde dehydrogenase 3 family, memberA1 ANGPT1 Angiopoietin 1 ANGPTL4 Angiopoietin-like 4 CASP8 Caspase 8, apoptosis-related cysteine peptidase CCNE2 Cyclin E2 CCR7 Chemokine (C-C motif) receptor 7 CD44 CD44 molecule (Indian blood group) CD82 CD82 antigen CDH1 Cadherin 1, type 1, E-cadherin (epithelial) CDH11 Cadherin 11, type 2, OB-cadherin (osteoblast) CDH2 Cadherin 2, type 1, N-cadherin CDH6 Cadherin 6 CLDN7 Claudin 7 COL1A1 Collagen, type I, alpha 1 COL4A2 Collagen, type IV, alpha 2 COL6A1 Collagen, type VI, alpha 1 CSF1 Colony stimulating factor 1 (macrophage) CSF3 Colony stimulating factor 3 (granulocyte) CST7 Cystatin F (leukocystatin) CTGF Connective tissue growth factor CTSB Cathepsin B CTSD Cathepsin D CTSK Cathepsin K CTSL1 Cathepsin L-like 3 CXCL1 Chemokine (C-X-C motif) ligand 1 CXCL12 Chemokine (C-X-C motif) ligand 12 CXCR4 Chemokine (C-X-C motif) receptor 4 CXCR6 Chemokine (C-X-C motif) receptor 6 DRG1 Developmentally regulated GTP binding protein 1 EREG Epiregulin FGF8 Fibroblast growth factor 8 (androgen-induced) FLT1 Fms-related tyrosine kinase FLT4 Fms-related tyrosine kinase 4 GPI Glucose phosphate isomerase GSN Gelsolin (amyloidosis, Finnish type) HGF Hepatocyte growth factor (hepapoietin A; scatter factor) HIF1A Hypoxia inducible factor 1, alpha subunit HMGB1 High-mobility group box 1 ID1 Inhibitor of DNA binding 1 IGFBP7 Insulin-like growth factor binding protein 7 IL13RA2 Interleukin 13 receptor, alpha 2 ISG20 Interferon stimulated exonuclease gene 20kDa JAG1 Jagged 1 (Alagille syndrome) KISS1 KiSS-1 metastasis-suppressor KLRC2 Killer cell lectin-like receptor subfamily C, member 2 KYNU Kynureninase (L-kynurenine hydrolase) LTBP1 Latent transforming growth factor beta binding protein 1 MAP2K4 Mitogen-activated protein kinase kinase 4 MAP2K5 Mitogen-activated protein kinase kinase 5 MAP2K7 Mitogen-activated protein kinase kinase 7 MCAM Melanoma cell adhesion molecule MET Met proto-oncogene (hepatocyte growth factor receptor) METAP2 Methionyl aminopeptidase 2 MMP1 Matrix metallopeptidase 1 (interstitial collagenase) MMP10 Matrix metallopeptidase 10 (stromelysin 2) MMP11 Matrix metallopeptidase 11 (stromelysin 3) MMP13 Matrix metallopeptidase 13 (collagenase 3) MMP14 Matrix metallopeptidase 14 (membrane-inserted) MMP2 Matrix metallopeptidase 2 MMP7 Matrix metallopeptidase 7 (matrilysin, uterine) MYC V-myc myelocytomatosis viral oncogene homolog NEDD9 Neural precursor cell expressed, dev. down- regulated 9 NF2 Neurofibromin 2 (merlin) NME1 Non-metastatic cells 1, protein (NM23A) NME2 Non-metastatic cells 2, protein NME4 Non-metastatic cells 4, protein PAX5 Paired box 5 PDGFA Platelet-derived growth factor alpha polypeptide PLAUR Plasminogen activator, urokinase receptor PTGS2 Prostaglandin-endoperoxide synthase 2 RUNX1 Runt-related transcription factor 1 SERPINE1 Serpin peptidase inhibitor, clade E SERPINB5 Serpin peptidase inhibitor, clade B5 SOX4 SRY (sex determining region Y)-box 4 SPARC Secreted protein, acidic, cysteine-rich (osteonectin) SPP1 Secreted phosphoprotein 1 SRC V-src sarcoma viral oncogene homolog (avian) SYK Spleen tyrosine kinase TFF1 Trefoil factor 1 TGFB1 Transforming growth factor, beta 1 TIMP1 TIMP metallopeptidase inhibitor 1 TIMP2 TIMP metallopeptidase inhibitor 2 TIMP3 TIMP metallopeptidase inhibitor 3 TIMP4 TIMP metallopeptidase inhibitor 4 TNC Tenascin C (hexabrachion) TP53 Tumor protein p53 VEGFA Vascular endothelial growth factor A ACTB Actin, beta B2M Beta-2-microglobulin GAPD Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $495.00 |
A ready-to-use Mouse Tumor Invasion/Metastasis PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against tumor invasion/metastasis genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: Adamts1 ADAM metallopeptidase Aldh3a1 Aldehyde dehydrogenase 3 family, memberA1 Angpt1 Angiopoietin 1 Angptl4 Angiopoietin-like 4 Casp8 Caspase 8, apoptosis-related cysteine peptidase Ccne2 Cyclin E2 Ccr7 Chemokine (C-C motif) receptor 7 Cd44 CD44 molecule (Indian blood group) Cd82 CD82 antigen Cdh1 Cadherin 1, type 1, E-cadherin (epithelial) Cdh11 Cadherin 11, type 2, OB-cadherin (osteoblast) Cdh2 Cadherin 2, type 1, N-cadherin Cdh6 Cadherin 6 Cldn7 Claudin 7 Col1a1 Collagen, type I, alpha 1 Col4a2 Collagen, type IV, alpha 2 Col6a1 Collagen, type VI, alpha 1 Csf1 Colony stimulating factor 1 (macrophage) Csf3 Colony stimulating factor 3 (granulocyte) Cst7 Cystatin F (leukocystatin) Ctgf Connective tissue growth factor Ctsb Cathepsin B Ctsd Cathepsin D Ctsk Cathepsin K Ctsl1 Cathepsin L-like 3 Cxcl1 Chemokine (C-X-C motif) ligand 1 Cxcl12 Chemokine (C-X-C motif) ligand 12 Cxcr4 Chemokine (C-X-C motif) receptor 4 Cxcr6 Chemokine (C-X-C motif) receptor 6 Drg1 Developmentally regulated GTP binding protein 1 Ereg Epiregulin Fgf8 Fibroblast growth factor 8 (androgen-induced) Flt1 Fms-related tyrosine kinase Flt4 Fms-related tyrosine kinase 4 Gpi Glucose phosphate isomerase Gsn Gelsolin (amyloidosis, Finnish type) Hgf Hepatocyte growth factor (hepapoietin A; scatter factor) Hif1a Hypoxia inducible factor 1, alpha subunit Hmgb1 High-mobility group box 1 Id1 Inhibitor of DNA binding 1 Igfbp7 Insulin-like growth factor binding protein 7 Il13ra2 Interleukin 13 receptor, alpha 2 Isg20 Interferon stimulated exonuclease gene 20kDa Jag1 Jagged 1 (Alagille syndrome) Kiss1 KiSS-1 metastasis-suppressor Klrc2 Killer cell lectin-like receptor subfamily C, member 2 Kynu Kynureninase (L-kynurenine hydrolase) Ltbp1 Latent transforming growth factor beta binding protein 1 Map2k4 Mitogen-activated protein kinase kinase 4 Map2k5 Mitogen-activated protein kinase kinase 5 Map2k7 Mitogen-activated protein kinase kinase 7 Mcam Melanoma cell adhesion molecule Met Met proto-oncogene (hepatocyte growth factor receptor) Metap2 Methionyl aminopeptidase 2 Mmp1 Matrix metallopeptidase 1 (interstitial collagenase) Mmp10 Matrix metallopeptidase 10 (stromelysin 2) Mmp11 Matrix metallopeptidase 11 (stromelysin 3) Mmp13 Matrix metallopeptidase 13 (collagenase 3) Mmp14 Matrix metallopeptidase 14 (membrane-inserted) Mmp2 Matrix metallopeptidase 2 Mmp7 Matrix metallopeptidase 7 (matrilysin, uterine) Myc V-myc myelocytomatosis viral oncogene homolog Nedd9 Neural precursor cell expressed, dev. down- regulated 9 Nf2 Neurofibromin 2 (merlin) Nme1 Non-metastatic cells 1, protein (NM23A) Nme2 Non-metastatic cells 2, protein Nme4 Non-metastatic cells 4, protein Pax5 Paired box 5 Pdgfa Platelet-derived growth factor alpha polypeptide Plaur Plasminogen activator, urokinase receptor Ptgs2 Prostaglandin-endoperoxide synthase 2 Runx1 Runt-related transcription factor 1 Serpine1 Serpin peptidase inhibitor, clade E Serpinb5 Serpin peptidase inhibitor, clade B5 Sox4 SRY (sex determining region Y)-box 4 Sparc Secreted protein, acidic, cysteine-rich (osteonectin) Spp1 Secreted phosphoprotein 1 Src V-src sarcoma viral oncogene homolog (avian) Syk Spleen tyrosine kinase Tff1 Trefoil factor 1 Tgfb1 Transforming growth factor, beta 1 Timp1 TIMP metallopeptidase inhibitor 1 Timp2 TIMP metallopeptidase inhibitor 2 Timp3 TIMP metallopeptidase inhibitor 3 Timp4 TIMP metallopeptidase inhibitor 4 Tnc Tenascin C (hexabrachion) Tp53 Tumor protein p53 Vegfa Vascular endothelial growth factor A Actb Actin, beta B2m Beta-2-microglobulin Gapd Glyceraldehyde-3-phosphate dehydrogenase Gusb Glucuronidase, beta Hprt1 Hypoxanthine phosphoribosyltransferase 1 Pgk Phosphoglycerate kinase 1 Ppia Peptidylprolyl isomerase A Rpl13a Ribosomal protein L13a $495.00 |