The advent of artificial intelligence has enabled the assembly of qPCR primer sets to amplify the transcripts associated with specific diseases, pathways, and processes. As an alternative to an empirical assembly of a gene list based on the literature, the AI program is able to evaluate the existing data and information to provide a list of the most relevant genes. The PCR primer sets for these genes are assembled into 96-well plates or libraries that may then be used to set-up PCR arrays. Each library may be used to generate up to 100 PCR arrays based on a 20ul reaction and a 0.1 uM final primer concentration.