Pre-designed human and mouse primer sets are $24.95 per pair, and rat sets are $29.95 per pair. Custom qPCR primer sets cost the same. You get a free primer set with any master mix order.
A 50 uM solution (40 ul) containing the forward and reverse primers, the primer sequences and detailed reaction conditions. That is enough for up to 1000 PCR reactions at a 20 ul reaction volume.
Human, mouse and rat primer sets are in the catalog. For other species, see "Other PCR Primer Sets" or email service@realtimeprimers.com.
Each set is designed against its target gene and optimized to produce clean PCR products with SYBR green-based real time PCR. Peer-reviewed papers that used our products are listed on our Publications/Citations page.
A PCR array is a 96- or 384-well plate of qPCR primer sets that measures a whole pathway in one SYBR green run. Our 96-well libraries have 88 pathway genes plus 8 housekeeping genes at 10 uM, 40 ul per set. That is up to 200 arrays at 10 ul, or about $2 per array (for example, the Human Apoptosis library is $495). We cover 100+ human, mouse and rat pathways, including Ferroptosis, ER Stress (UPR) and Apoptosis.
Yes. They are built for the same pathway-focused experiments, but one library supports many arrays instead of one sample per plate. (RT2 Profiler is a trademark of Qiagen.)
Primer Bot is a free AI tool on our AI PCR Arrays page. Type a pathway or disease to build a gene list of up to 96 or 384 genes, then request a quote or order a custom library.
Yes. We sell AzuraQuant Green and Probe Fast qPCR master mixes (Fluor, LoRox and HiRox versions), 1-step qRT-PCR kits, and AzuraQuant cDNA Synthesis Kits in sizes from 25 to 1000 reactions.
Yes. Email service@realtimeprimers.com with your P.O. number, shipping address, billing address and a phone number for delivery. You can also call (855) PRIMERS / (215) 964-3274 or fax (215) 689-3768. A W-9 and vendor information are available on request. International customers can order through our local distributors.