A ready-to-use Mouse Cuproptosis PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against cuproptosis genes and 8 housekeeping gene primer sets.
Provided in a 96-well microplate (20 ul - 10 uM)
Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)
Just add cDNA template and SYBR green master mix
GENE LIST:
Abca7 ATP-binding cassette, sub-family A member 7
Adam17 a disintegrin and metallopeptidase domain 17
Adam9 a disintegrin and metallopeptidase domain 9
Afp alpha fetoprotein
Alb albumin
App amyloid beta precursor protein
Aqp1 aquaporin 1
Atox1 antioxidant 1 copper chaperone
Atp7a ATPase, Cu++ transporting, alpha polypeptide
Atp7b ATPase, Cu++ transporting, beta polypeptide
Bcl3 B cell leukemia/lymphoma 3
Bcs1l BCS1-like (yeast)
Bin1 bridging integrator 1
Birc2 baculoviral IAP repeat-containing 2
Btla B and T lymphocyte associated
Casp2 caspase 2
Cd274 CD274 antigen (PD-L1)
Cd2ap CD2-associated protein
Cd33 CD33 antigen
Cdk1 cyclin-dependent kinase 1
Cdkn2a cyclin dependent kinase inhibitor 2A
Celf1 CUGBP, Elav-like family member 1
Clu clusterin
Coa6 cytochrome c oxidase assembly factor 6
Cox11 cytochrome c oxidase assembly protein 11
Cox17 cytochrome c oxidase assembly protein 17, copper chaperone
Cox19 cytochrome c oxidase assembly protein 19
Chchd7 coiled-coil-helix-coiled-coil-helix domain containing 7 (COX23)
Cp ceruloplasmin
CT47A1 cancer/testis antigen family 47 member A1 (human gene, no mouse ortholog)
Slc31a1 solute carrier family 31, member 1 (CTR1)
Dbt dihydrolipoamide branched chain transacylase E2
Dlat dihydrolipoamide S-acetyltransferase
Dld dihydrolipoamide dehydrogenase
Dlst dihydrolipoamide S-succinyltransferase
Dnm1 dynamin 1
Epha1 Eph receptor A1
Fadd Fas (TNFRSF6)-associated via death domain
Fdx1 ferredoxin 1
Gcsh glycine cleavage system protein H (aminomethyl carrier)
Gls glutaminase
Gpc1 glypican 1
Gpx4 glutathione peroxidase 4
Inpp5d inositol polyphosphate-5-phosphatase D
Iscu iron-sulfur cluster assembly enzyme
Itgb6 integrin beta 6
Kcna3 potassium voltage-gated channel, shaker-related subfamily, member 3
Lias lipoic acid synthetase
Lipt1 lipoyltransferase 1
Lox lysyl oxidase
Loxl2 lysyl oxidase-like 2
Map2k1 mitogen-activated protein kinase kinase 1
Mapt microtubule-associated protein tau
Mef2c myocyte enhancer factor 2C
Ms4a6b membrane-spanning 4-domains, subfamily A, member 6B
Mt2 metallothionein 2
mt-Co1 mitochondrially encoded cytochrome c oxidase I
mt-Co2 mitochondrially encoded cytochrome c oxidase II
Mtf1 metal response element binding transcription factor 1
Ndufc1 NADH:ubiquinone oxidoreductase subunit C1
Nfu1 NFU1 iron-sulfur cluster scaffold
Nploc4 NPL4 homolog, ubiquitin recognition factor
Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase
Pam peptidylglycine alpha-amidating monooxygenase
Pde3b phosphodiesterase 3B, cGMP-inhibited
Pdha1 pyruvate dehydrogenase E1 alpha 1
Pdhb pyruvate dehydrogenase (lipoamide) beta
Pdhx pyruvate dehydrogenase complex, component X
Pdk1 pyruvate dehydrogenase kinase, isoenzyme 1
Pdx1 pancreatic and duodenal homeobox 1
Picalm phosphatidylinositol binding clathrin assembly protein
Prrx1 paired related homeobox 1
Psen1 presenilin 1
Ptk2b PTK2 protein tyrosine kinase 2 beta
Sco1 SCO1 cytochrome c oxidase assembly protein
Sco2 SCO2 cytochrome c oxidase assembly protein
Sirt2 sirtuin 2
Slc31a1 solute carrier family 31, member 1
Slc31a2 solute carrier family 31, member 2
Sod1 superoxide dismutase 1, soluble
Sod2 superoxide dismutase 2, mitochondrial
Sod3 superoxide dismutase 3, extracellular
Sorl1 sortilin-related receptor, LDLR class A repeats-containing
Steap3 STEAP family member 3
Tmprss6 transmembrane serine protease 6
Tradd TNFRSF1A-associated via death domain
Trem2 triggering receptor expressed on myeloid cells 2
Vcp valosin containing protein
Actb Actin, beta
B2m Beta-2-microglobulin
Gapdh Glyceraldehyde-3-phosphate dehydrogenase
Gusb Glucuronidase, beta
Hprt Hypoxanthine phosphoribosyltransferase 1
Pgk1 Phosphoglycerate kinase 1
Ppia Peptidylprolyl isomerase A
Rpl13a Ribosomal protein L13a