Human Cell Cycle Primer Library

Contains 88 primer sets directed against cell cycle genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ABL1 V-abl Abelson murine leukemia viral oncogene homolog 1

ANAPC2 Anaphase promoting complex subunit 2

ANAPC4 Anaphase promoting complex subunit 4

ATM Ataxia telangiectasia mutated (includes complementation groups A, C and D)

ATR Ataxia telangiectasia and Rad3 related

BAX BCL2-associated X protein

BCCIP BRCA2 and CDKN1A interacting protein

BCL2 B-cell CLL/lymphoma 2

BIRC5 Baculoviral IAP repeat-containing 5 (survivin)

BRCA1 Breast cancer 1, early onset

BRCA2 Breast cancer 2, early onset

CCNA1 Cyclin A1

CCNA2 Cyclin A2

CCNB1 Cyclin B1

CCNB2 Cyclin B2

CCNC Cyclin C

CCND1 Cyclin D1

CCND2 Cyclin D2

CCNE1 Cyclin E1

CCNE2 Cyclin E2

CCNF Cyclin F

CCNG1 Cyclin G1

CCNG2 Cyclin G2

CCNH Cyclin H

CCNT1 Cyclin T1

CDC16 Cell division cycle 16 homolog (S. cerevisiae)

CDC2 Cell division cycle 2, G1 to S and G2 to M

CDC20 Cell division cycle 20 homolog (S. cerevisiae)

CDC34 Cell division cycle 34 homolog (S. cerevisiae)

CDK2 Cyclin-dependent kinase 2

CDK4 Cyclin-dependent kinase 4

CDK5 Cyclin-dependent kinase 5

CDK5R1 Cyclin-dependent kinase 5, regulatory subunit 1 (p35)

CDK5RAP1 CDK5 regulatory subunit associated protein 1

CDK6 Cyclin-dependent kinase 6

CDK7 Cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase)

CDK8 Cyclin-dependent kinase 8

CDKN1A Cyclin-dependent kinase inhibitor 1A (p21, Cip1)

CDKN1B-2 Cyclin-dependent kinase inhibitor 1B (p27, Kip1)

CDKN2A-3 Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

CDKN2B Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)

CDKN2C Cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)

CDKN2D Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)

CDKN3 Cyclin-dependent kinase inhibitor 3 (CDK2- associated dual specificity phosphatase)

CHEK1 CHK1 checkpoint homolog (S. pombe)

CHEK2 CHK2 checkpoint homolog (S. pombe)

CKS1B CDC28 protein kinase regulatory subunit 1B

CKS2 CDC28 protein kinase regulatory subunit 2

CUL1 Cullin 1

CUL2 Cullin 2

CUL3 Cullin 3

DDX11 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae)

DIRAS3 DIRAS family, GTP-binding RAS-like 3

DNM2 Dynamin 2

E2F4 E2F transcription factor 4, p107/p130-binding

GADD45A-6 Growth arrest and DNA-damage-inducible, alpha

GTF2H1 General transcription factor IIH, polypeptide 1, 62kDa

GTSE1 G-2 and S-phase expressed 1

HERC5 Hect domain and RLD 5

HUS1 HUS1 checkpoint homolog (S. pombe)

KNTC1 Kinetochore associated 1

KPNA2 Karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

MAD2L1 MAD2 mitotic arrest deficient-like 1 (yeast)

MAD2L2 MAD2 mitotic arrest deficient-like 2 (yeast)

MCM2 MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)

MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)

MCM4 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)

MCM5 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)

MKI67 Antigen identified by monoclonal antibody Ki-67

MNAT1 Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)

MRE11A MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

NBN Nibrin

PCNA Proliferating cell nuclear antigen

RAD1 RAD1 homolog (S. pombe)

RAD17 RAD17 homolog (S. pombe)

RAD51 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)

RB1 Retinoblastoma 1 (including osteosarcoma)

RBBP8 Retinoblastoma binding protein 8

RBL1 Retinoblastoma-like 1 (p107)

RBL2 Retinoblastoma-like 2 (p130)

RPA3 Replication protein A3, 14kDa

SERTAD1 SERTA domain containing 1

SKP2 S-phase kinase-associated protein 2 (p45)

SUMO1 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)

SUMO2 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)

TFDP2 Transcription factor Dp-2 (E2F dimerization partner 2)

TP53 Tumor protein p53 (Li-Fraumeni syndrome)

UBE1 Ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing)

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a