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Cell Cycle

The cell cycle is the ordered series of events a eukaryotic cell goes through to grow, duplicate its genetic material, and divide into two new daughter cells. Its primary function is to accurately copy the cell's vast amount of DNA and precisely segregate the copies into two genetically identical daughter cells. The cell cycle is divided into two main phases: Interphase (growth and DNA replication) and the Mitotic Phase (cell division). The cell cycle is highly regulated by a control system to ensure events occur in the correct sequence and only when conditions are right. This control relies on checkpoints and key regulatory molecules. The progression through the cycle is primarily driven by the fluctuating activity of two types of proteins: Cyclins are proteins whose concentrations change (increase and decrease) throughout the cell cycle. Cyclin-Dependent Kinases (CDKs): Enzymes whose activity depends on binding to a specific cyclin. The active CDK complex phosphorylates target proteins, triggering the events of the next cell cycle stage.

Human Cell Cycle Primer Library
A ready-to-use Human Cell Cycle PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against cell cycle genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ABL1 V-abl Abelson murine leukemia viral oncogene homolog 1

ANAPC2 Anaphase promoting complex subunit 2

ANAPC4 Anaphase promoting complex subunit 4

ATM Ataxia telangiectasia mutated (includes complementation groups A, C and D)

ATR Ataxia telangiectasia and Rad3 related

BAX BCL2-associated X protein

BCCIP BRCA2 and CDKN1A interacting protein

BCL2 B-cell CLL/lymphoma 2

BIRC5 Baculoviral IAP repeat-containing 5 (survivin)

BRCA1 Breast cancer 1, early onset

BRCA2 Breast cancer 2, early onset

CCNA1 Cyclin A1

CCNA2 Cyclin A2

CCNB1 Cyclin B1

CCNB2 Cyclin B2

CCNC Cyclin C

CCND1 Cyclin D1

CCND2 Cyclin D2

CCNE1 Cyclin E1

CCNE2 Cyclin E2

CCNF Cyclin F

CCNG1 Cyclin G1

CCNG2 Cyclin G2

CCNH Cyclin H

CCNT1 Cyclin T1

CDC16 Cell division cycle 16 homolog (S. cerevisiae)

CDC2 Cell division cycle 2, G1 to S and G2 to M

CDC20 Cell division cycle 20 homolog (S. cerevisiae)

CDC34 Cell division cycle 34 homolog (S. cerevisiae)

CDK2 Cyclin-dependent kinase 2

CDK4 Cyclin-dependent kinase 4

CDK5 Cyclin-dependent kinase 5

CDK5R1 Cyclin-dependent kinase 5, regulatory subunit 1 (p35)

CDK5RAP1 CDK5 regulatory subunit associated protein 1

CDK6 Cyclin-dependent kinase 6

CDK7 Cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase)

CDK8 Cyclin-dependent kinase 8

CDKN1A Cyclin-dependent kinase inhibitor 1A (p21, Cip1)

CDKN1B-2 Cyclin-dependent kinase inhibitor 1B (p27, Kip1)

CDKN2A-3 Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

CDKN2B Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)

CDKN2C Cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)

CDKN2D Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)

CDKN3 Cyclin-dependent kinase inhibitor 3 (CDK2- associated dual specificity phosphatase)

CHEK1 CHK1 checkpoint homolog (S. pombe)

CHEK2 CHK2 checkpoint homolog (S. pombe)

CKS1B CDC28 protein kinase regulatory subunit 1B

CKS2 CDC28 protein kinase regulatory subunit 2

CUL1 Cullin 1

CUL2 Cullin 2

CUL3 Cullin 3

DDX11 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae)

DIRAS3 DIRAS family, GTP-binding RAS-like 3

DNM2 Dynamin 2

E2F4 E2F transcription factor 4, p107/p130-binding

GADD45A-6 Growth arrest and DNA-damage-inducible, alpha

GTF2H1 General transcription factor IIH, polypeptide 1, 62kDa

GTSE1 G-2 and S-phase expressed 1

HERC5 Hect domain and RLD 5

HUS1 HUS1 checkpoint homolog (S. pombe)

KNTC1 Kinetochore associated 1

KPNA2 Karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

MAD2L1 MAD2 mitotic arrest deficient-like 1 (yeast)

MAD2L2 MAD2 mitotic arrest deficient-like 2 (yeast)

MCM2 MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)

MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)

MCM4 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)

MCM5 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)

MKI67 Antigen identified by monoclonal antibody Ki-67

MNAT1 Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)

MRE11A MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

NBN Nibrin

PCNA Proliferating cell nuclear antigen

RAD1 RAD1 homolog (S. pombe)

RAD17 RAD17 homolog (S. pombe)

RAD51 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)

RB1 Retinoblastoma 1 (including osteosarcoma)

RBBP8 Retinoblastoma binding protein 8

RBL1 Retinoblastoma-like 1 (p107)

RBL2 Retinoblastoma-like 2 (p130)

RPA3 Replication protein A3, 14kDa

SERTAD1 SERTA domain containing 1

SKP2 S-phase kinase-associated protein 2 (p45)

SUMO1 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)

SUMO2 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)

TFDP2 Transcription factor Dp-2 (E2F dimerization partner 2)

TP53 Tumor protein p53 (Li-Fraumeni syndrome)

UBE1 Ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing)

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPD Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a
$495.00
HCC-I
Mouse Cell Cycle Primer Library
A ready-to-use Mouse Cell Cycle PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against cell cycle genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

Abl1 V-abl Abelson murine leukemia viral oncogene homolog 1

Anapc2 Anaphase promoting complex subunit 2

Anapc4 Anaphase promoting complex subunit 4

Atm Ataxia telangiectasia mutated (includes complementation groups A, C and D)

Atr Ataxia telangiectasia and Rad3 related

Bax BCL2-associated X protein

Bccip BRCA2 and CDKN1A interacting protein

Bcl2 B-cell CLL/lymphoma 2

Birc5 Baculoviral IAP repeat-containing 5 (survivin)

Brca1 Breast cancer 1, early onset

Brca2 Breast cancer 2, early onset

Ccna1 Cyclin A1

Ccna2 Cyclin A2

Ccnb1 Cyclin B1

Ccnb2 Cyclin B2

Ccnc Cyclin C

Ccnd1 Cyclin D1

Ccnd2 Cyclin D2

Ccne1 Cyclin E1

Ccne2 Cyclin E2

Ccnf Cyclin F

Ccng1 Cyclin G1

Ccng2 Cyclin G2

Ccnh Cyclin H

Ccnt1 Cyclin T1

Cdc16 Cell division cycle 16 homolog (S. cerevisiae)

Cdc2 Cell division cycle 2, G1 to S and G2 to M

Cdc20 Cell division cycle 20 homolog (S. cerevisiae)

Cdc34 Cell division cycle 34 homolog (S. cerevisiae)

Cdk2 Cyclin-dependent kinase 2

Cdk4 Cyclin-dependent kinase 4

Cdk5 Cyclin-dependent kinase 5

Cdk5R1 Cyclin-dependent kinase 5, regulatory subunit 1 (p35)

Cdk5Rap1 CDK5 regulatory subunit associated protein 1

Cdk6 Cyclin-dependent kinase 6

Cdk7 Cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase)

Cdk8 Cyclin-dependent kinase 8

Cdkn1A Cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Cdkn1B Cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Cdkn1C Cyclin-dependent kinase inhibitor 1c

Cdkn2A Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Cdkn2B Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)

Cdkn2C Cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)

Cdkn2D Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)

Cdkn3 Cyclin-dependent kinase inhibitor 3 (CDK2- associated dual specificity phosphatase)

Chek1 CHK1 checkpoint homolog (S. pombe)

Chek2 CHK2 checkpoint homolog (S. pombe)

Cks1B CDC28 protein kinase regulatory subunit 1B

Cks2 CDC28 protein kinase regulatory subunit 2

Cul1 Cullin 1

Cul2 Cullin 2

Cul3 Cullin 3

Ddx11 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae)

Dnm2 Dynamin 2

E2f1 E2F transcription factor 1

E2f2 E2F transcription factor 2

E2F4 E2F transcription factor 4, p107/p130-binding

Gadd45A Growth arrest and DNA-damage-inducible, alpha

Gtf2H1 General transcription factor IIH, polypeptide 1, 62kDa

Gtse1 G-2 and S-phase expressed 1

Hus1 HUS1 checkpoint homolog (S. pombe)

Kntc1 Kinetochore associated 1

Kpna2 Karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Mad2L1 MAD2 mitotic arrest deficient-like 1 (yeast)

Mad2L2 MAD2 mitotic arrest deficient-like 2 (yeast)

Mcm2 MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)

Mcm3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)

Mcm4 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)

Mcm5 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)

Mki67 Antigen identified by monoclonal antibody Ki-67

Mnat1 Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)

Mre11A MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

Nbn Nibrin

Pcna Proliferating cell nuclear antigen

Rad1 RAD1 homolog (S. pombe)

Rad17 RAD17 homolog (S. pombe)

Rad51 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)

Rb1 Retinoblastoma 1 (including osteosarcoma)

Rbbp8 Retinoblastoma binding protein 8

Rbl1 Retinoblastoma-like 1 (p107)

Rbl2 Retinoblastoma-like 2 (p130)

Rpa3 Replication protein A3, 14kDa

Sertad1 SERTA domain containing 1

Skp2 S-phase kinase-associated protein 2 (p45)

Sumo1 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)

Sumo2 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)

Tfdp2 Transcription factor Dp-2 (E2F dimerization partner 2)

Trp53 Tumor protein p53 (Li-Fraumeni syndrome)

Actb Actin, beta

B2m Beta-2-microglobulin

Gapd Glyceraldehyde-3-phosphate dehydrogenase

Gusb Glucuronidase, beta

Hprt1 Hypoxanthine phosphoribosyltransferase 1

Pgk1 Phosphoglycerate kinase 1

Ppia Peptidylprolyl isomerase A

Rpl13a Ribosomal protein L13a

$495.00
MCC-I