SYBR Green qPCR primers for detecting rat Sparc (secreted protein acidic and cysteine rich, also called ON) gene expression by real-time PCR.
Rat Sparc qPCR Primer Set – secreted protein acidic and cysteine rich
About this gene: Sparc enables calcium ion binding activity. It is involved in multiple processes, including response to L-ascorbic acid; response to lead ion; and response to lipopolysaccharide. Is active in glutamatergic synapse.
Gene details: NCBI Gene ID: 24791; UniProt: P16975; Chromosome location: 10q22
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.