SYBR Green qPCR primers for detecting rat Spag9 (sperm associated antigen 9) gene expression by real-time PCR.
Rat Spag9 qPCR Primer Set – sperm associated antigen 9
About this gene: Spag9 is predicted to enable multiple functions, including kinesin binding activity; protein kinase binding activity; and signaling adaptor activity. It is involved in negative regulation of dendrite extension; negative regulation of neuron differentiation; and negative regulation of protein phosphorylation. Predicted to be active in cytoplasm.
Gene details: NCBI Gene ID: 360600; Chromosome location: 10q26
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.