SYBR Green qPCR primers for detecting rat Atp6v1d (ATPase H+ transporting V1 subunit D) gene expression by real-time PCR.
Rat Atp6v1d qPCR Primer Set – ATPase H+ transporting V1 subunit D
About this gene: Predicted to contribute to proton-transporting ATPase activity, rotational mechanism. It is predicted to be involved in multiple processes, including protein localization to cilium; synaptic vesicle lumen acidification; and vacuolar acidification. Is extrinsic component of synaptic vesicle membrane.
Gene details: NCBI Gene ID: 299159; Chromosome location: 6q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.