SYBR Green qPCR primers for detecting rat Asip (agouti signaling protein, also called ASP) gene expression by real-time PCR.
Rat Asip qPCR Primer Set – agouti signaling protein
Also known as: ASP
About this gene: Asip is involved in hair pigmentation. In multiple strains Asip encodes a functional protein while in others it lacks a 19 nt indel and is non-protein coding.
Gene details: NCBI Gene ID: 24152; UniProt: Q99JA2; Chromosome location: 3q41
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.