SYBR Green qPCR primers for detecting rat Madd (MAP-kinase activating death domain, also called RabGEF) gene expression by real-time PCR.
Rat Madd qPCR Primer Set – MAP-kinase activating death domain
Also known as: RabGEF, RabGEP
About this gene: Madd enables guanyl-nucleotide exchange factor activity. It is involved in negative regulation of growth hormone secretion and negative regulation of pancreatic amylase secretion. Is active in synapse.
Gene details: NCBI Gene ID: 94193; UniProt: O08873; Chromosome location: 3q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.