SYBR Green qPCR primers for detecting rat Lpcat1 (lysophosphatidylcholine acyltransferase 1, also called LysoPAFAT) gene expression by real-time PCR.
Rat Lpcat1 qPCR Primer Set – lysophosphatidylcholine acyltransferase 1
Also known as: Aytl2, RGD1311599
About this gene: Lpcat1 enables 1-acylglycerol-3-phosphate O-acyltransferase activity and 1-acylglycerophosphocholine O-acyltransferase activity. It is predicted to be involved in phosphatidylcholine acyl-chain remodeling and phospholipid biosynthetic process. It is predicted to act upstream of or within multiple processes, including negative regulation of phosphatidylcholine biosynthetic process; positive regulation of protein catabolic process; and surfactant homeostasis.
Gene details: NCBI Gene ID: 361467; UniProt: Q1HAQ0; Chromosome location: 1p11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.