SYBR Green qPCR primers for detecting rat Lhx5 (LIM homeobox 5) gene expression by real-time PCR.
Rat Lhx5 qPCR Primer Set – LIM homeobox 5
About this gene: Lhx5 is predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. It is predicted to be involved in positive regulation of DNA-templated transcription; regulation of transcription by RNA polymerase II; and spinal cord association neuron differentiation. It is predicted to act upstream of or within brain development; cerebellar Purkinje cell-granule cell precursor cell signaling; and regulation of cell population proliferation.
Gene details: NCBI Gene ID: 124451; UniProt: P61376; Chromosome location: 12q16
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.