SYBR Green qPCR primers for detecting rat Kcna1 (potassium voltage-gated channel subfamily A member 1, also called RBKI) gene expression by real-time PCR.
Rat Kcna1 qPCR Primer Set – potassium voltage-gated channel subfamily A member 1
Also known as: Kcna, Kcpvd, Kv1.1
About this gene: Kcna1 enables delayed rectifier potassium channel activity; voltage-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential; and voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential. It is involved in multiple processes, including nervous system development; neuronal action potential; and neuronal signal transduction. Is active in calyx of Held; glutamatergic synapse; and synaptic membrane.
Gene details: NCBI Gene ID: 24520; UniProt: P10499; Chromosome location: 4q42
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.