SYBR Green qPCR primers for detecting rat Irs2 (insulin receptor substrate 2) gene expression by real-time PCR.
Rat Irs2 qPCR Primer Set – insulin receptor substrate 2
Also known as: 4PS, IRS-2
About this gene: Irs2 enables multiple functions, including 14-3-3 protein binding activity; insulin receptor binding activity; and phosphatidylinositol 3-kinase binding activity. It is involved in cellular response to endothelin; cellular response to glucose stimulus; and insulin receptor signaling pathway. Predicted to be active in cytosol and plasma membrane.
Gene details: NCBI Gene ID: 29376; Chromosome location: 16q12.5
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.