SYBR Green qPCR primers for detecting rat Irf3 (interferon regulatory factor 3) gene expression by real-time PCR.
Rat Irf3 qPCR Primer Set – interferon regulatory factor 3
About this gene: Irf3 is predicted to enable multiple functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; promoter-specific chromatin binding activity; and protein homodimerization activity. It is involved in cellular response to exogenous dsRNA; positive regulation of canonical NF-kappaB signal transduction; and positive regulation of cytokine production. Predicted to be active in chromatin; cytoplasm; and nucleus.
Gene details: NCBI Gene ID: 292892; Chromosome location: 1q22
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.