SYBR Green qPCR primers for detecting rat Hyal1 (hyaluronidase 1) gene expression by real-time PCR.
Rat Hyal1 qPCR Primer Set – hyaluronidase 1
About this gene: Hyal1 is predicted to enable chondroitin hydrolase activity and hyalurononglucosaminidase activity. It is predicted to be involved in multiple processes, including cellular response to UV-B; cellular response to cytokine stimulus; and regulation of cell growth. It is predicted to act upstream of or within embryonic skeletal joint morphogenesis.
Gene details: NCBI Gene ID: 367166; UniProt: Q76HN1; Chromosome location: 8q32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.