SYBR Green qPCR primers for detecting rat Htr2a (5-hydroxytryptamine receptor 2A) gene expression by real-time PCR.
Rat Htr2a qPCR Primer Set – 5-hydroxytryptamine receptor 2A
Also known as: 5-HT2A, 5Ht-2
About this gene: Htr2a is predicted to enable multiple functions, including 1-propan-2-amine binding activity; Gq/11-coupled serotonin receptor activity; and serotonin binding activity. It is involved in multiple processes, including blood vessel diameter maintenance; positive regulation of MAPK cascade; and sensory perception of pain. Is active in glutamatergic synapse; postsynaptic membrane; and presynaptic membrane.
Gene details: NCBI Gene ID: 29595; UniProt: P14842; Chromosome location: 15q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.