SYBR Green qPCR primers for detecting rat Hoxd9 (homeo box D9) gene expression by real-time PCR.
Rat Hoxd9 qPCR Primer Set – homeo box D9
About this gene: Hoxd9 is predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. It is predicted to be involved in embryonic skeletal system morphogenesis; negative regulation of transcription by RNA polymerase II; and regionalization. It is predicted to act upstream of or within multiple processes, including embryonic skeletal system development; limb morphogenesis; and peripheral nervous system neuron development.
Gene details: NCBI Gene ID: 688999; UniProt: B5DFK3; Chromosome location: 3q23
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.