SYBR Green qPCR primers for detecting rat Hmgcs1 (3-hydroxy-3-methylglutaryl-CoA synthase 1) gene expression by real-time PCR.
Rat Hmgcs1 qPCR Primer Set – 3-hydroxy-3-methylglutaryl-CoA synthase 1
About this gene: Hmgcs1 enables hydroxymethylglutaryl-CoA synthase activity; isomerase activity; and organic acid binding activity. Predicted to be active in cytosol.
Gene details: NCBI Gene ID: 29637; UniProt: P17425; Chromosome location: 2q15
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.