SYBR Green qPCR primers for detecting rat Hmgcr (3-hydroxy-3-methylglutaryl-CoA reductase) gene expression by real-time PCR.
Rat Hmgcr qPCR Primer Set – 3-hydroxy-3-methylglutaryl-CoA reductase
Also known as: 3H3M
About this gene: Hmgcr enables identical protein binding activity and protein phosphatase 2A binding activity. It is involved in multiple processes, including cholesterol homeostasis; positive regulation of MAPK cascade; and regulation of striated muscle cell apoptotic process. Predicted to be active in cytoplasmic side of endoplasmic reticulum membrane and peroxisomal membrane.
Gene details: NCBI Gene ID: 25675; UniProt: P51639; Chromosome location: 2q12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.