SYBR Green qPCR primers for detecting rat Hmgcl (3-hydroxy-3-methylglutaryl-CoA lyase, also called HL) gene expression by real-time PCR.
Rat Hmgcl qPCR Primer Set – 3-hydroxy-3-methylglutaryl-CoA lyase
About this gene: Hmgcl enables carboxylic acid binding activity; fatty-acyl-CoA binding activity; and hydroxymethylglutaryl-CoA lyase activity. It is involved in multiple processes, including ketone body biosynthetic process; liver development; and response to fatty acid. Predicted to be active in mitochondrion.
Gene details: NCBI Gene ID: 79238; UniProt: P97519; Chromosome location: 5q36
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.