SYBR Green qPCR primers for detecting rat Foxa2 (forkhead box A2, also called HNF-3B) gene expression by real-time PCR.
Rat Foxa2 qPCR Primer Set – forkhead box A2
Also known as: Hnf3b
About this gene: Foxa2 enables multiple functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; SMAD binding activity; and transcription corepressor activity. It is involved in negative regulation of transcription by RNA polymerase II; positive regulation of transcription by RNA polymerase II; and response to insulin.
Gene details: NCBI Gene ID: 25099; UniProt: P32182; Chromosome location: 3q41
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.