SYBR Green qPCR primers for detecting rat Fads2 (fatty acid desaturase 2, also called D6D) gene expression by real-time PCR.
Rat Fads2 qPCR Primer Set – fatty acid desaturase 2
Also known as: Fadsd6
About this gene: Fads2 is predicted to enable acyl-CoA 6-desaturase activity and stearoyl-CoA 9-desaturase activity. It is involved in arachidonate metabolite production involved in inflammatory response and positive regulation of cellular response to oxidative stress. Predicted to be active in endoplasmic reticulum membrane.
Gene details: NCBI Gene ID: 83512; UniProt: Q9Z122; Chromosome location: 1q43
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.