SYBR Green qPCR primers for detecting rat E4f1 (E4F transcription factor 1) gene expression by real-time PCR.
Rat E4f1 qPCR Primer Set – E4F transcription factor 1
Also known as: Dnase1l2
About this gene: E4f1 is predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II-specific DNA-binding transcription factor binding activity; and cAMP response element binding activity. It is predicted to be involved in negative regulation of transcription by RNA polymerase II and regulation of cell cycle process. It is predicted to act upstream of or within DNA replication; positive regulation of transcription by RNA polymerase II; and regulation of mitotic cell cycle, embryonic.
Gene details: NCBI Gene ID: 681359; Chromosome location: 10q12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.