SYBR Green qPCR primers for detecting rat Cry1 (cryptochrome circadian regulator 1) gene expression by real-time PCR.
Rat Cry1 qPCR Primer Set – cryptochrome circadian regulator 1
About this gene: Cry1 encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. Cry1 is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in Cry1 have been linked to altered sleep patterns.
Gene details: NCBI Gene ID: 299691; UniProt: Q32Q86; Chromosome location: 7q13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.