SYBR Green qPCR primers for detecting rat Crb3 (crumbs cell polarity complex component 3) gene expression by real-time PCR.
Rat Crb3 qPCR Primer Set – crumbs cell polarity complex component 3
About this gene: Crb3 is predicted to enable SH3 domain binding activity. It is predicted to be involved in positive regulation of cell junction assembly; protein localization to plasma membrane; and tight junction organization.
Gene details: NCBI Gene ID: 301112; Chromosome location: 9q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.