SYBR Green qPCR primers for detecting mouse Capzb (capping actin protein of muscle Z-line subunit beta) gene expression by real-time PCR.
Mouse Capzb qPCR Primer Set – capping actin protein of muscle Z-line subunit beta
Also known as: 1700120C01Rik, CPB1, CPB2, CPbeat2, CPbeta1, CPbeta2, Cappb1
About this gene: Capzb encodes a beta subunit of a highly conserved filamentous actin capping protein that binds the barbed end of filamentous actin to stabilize it and terminate elongation. Interaction of this protein with the barbed end of the actin filament occurs through binding of the amphipathic helix at the C-terminus to the hydrophobic cleft on the actin molecule. Capzb is required for a variety of dynamic actin-mediated processes including organization of lamellipodia and filopodia, growth cone morphology and neurite outgrowth in hippocampal neurons, and asymmetric spindle migration and polar body extrusion during oocyte maturation.
Gene details: NCBI Gene ID: 12345; UniProt: P47757; Chromosome location: 4 D3|4 70.59 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.