SYBR Green qPCR primers for detecting mouse Wt1 (WT1 transcription factor) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Wt1 qPCR Primer Set – WT1 transcription factor
Also known as: D630046I19Rik, Wt-1
About this gene: Wt1 encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. The Wt1 gene product plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. The mRNA for Wt1 is reported to initiate translation from non-AUG and AUG translation start sites, resulting in different isoforms.
Gene details: NCBI Gene ID: 22431; Chromosome location: 2 E3|2 55.06 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.