SYBR Green qPCR primers for detecting mouse Bsn (bassoon) gene expression by real-time PCR.
Mouse Bsn qPCR Primer Set – bassoon
About this gene: A structural constituent of presynaptic active zone. It is involved in protein localization to synapse; regulation of synaptic vesicle cycle; and synaptic vesicle clustering. Is active in multiple cellular components, including GABA-ergic synapse; Schaffer collateral - CA1 synapse; and cochlear hair cell ribbon synapse.
Gene details: NCBI Gene ID: 12217; UniProt: O88737; Chromosome location: 9 F1|9 59.07 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.