SYBR Green qPCR primers for detecting mouse Twist1 (twist basic helix-loop-helix transcription factor 1, also called M-twist) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Twist1 qPCR Primer Set – twist basic helix-loop-helix transcription factor 1
Also known as: M-Twist, Pde, Ska10, Ska<m10Jus>, Twist, bHLHa38, pdt
About this gene: Basic helix-loop-helix transcription factors have been implicated in cell lineage determination and differentiation. Twist1 encodes a bHLH transcription factor that is evolutionarily conserved from invertebrates to humans, and was originally identified in Drosophila as an essential gene involved in early mesoderm development and dorsal-ventral patterning in the embryo. The Twist1 protein is involved in cancer by regulating the epithelial-mesenchymal transition, a process that is critical for metastasis initiation, and promoting tumor progression.
Gene details: NCBI Gene ID: 22160; UniProt: P26687; Chromosome location: 12 A3|12 14.81 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.