SYBR Green qPCR primers for detecting mouse Trim8 (tripartite motif-containing 8) gene expression by real-time PCR.
Mouse Trim8 qPCR Primer Set – tripartite motif-containing 8
Also known as: GERP, Rnf27
About this gene: Trim8 is predicted to enable multiple functions, including mitogen-activated protein kinase kinase kinase binding activity; protein homodimerization activity; and transcription coactivator activity. It is involved in host-mediated suppression of symbiont invasion; negative regulation of viral transcription; and suppression of viral release by host. It acts upstream of or within positive regulation of protein localization to nucleus and stem cell population maintenance.
Gene details: NCBI Gene ID: 93679; UniProt: Q99PJ2; Chromosome location: 19 C3|19 38.92 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.