SYBR Green qPCR primers for detecting mouse Acadm (acyl-Coenzyme A dehydrogenase, medium chain, also called MCAD) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Acadm qPCR Primer Set – acyl-Coenzyme A dehydrogenase, medium chain
Also known as: MCAD
About this gene: Acadm encodes a homotetrameric mitochondrial flavoprotein and belongs to the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length.
Gene details: NCBI Gene ID: 11364; UniProt: P45952; Chromosome location: 3 H3|3 78.77 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.