SYBR Green qPCR primers for detecting mouse Sqle (squalene epoxidase, also called SE) gene expression by real-time PCR.
Mouse Sqle qPCR Primer Set – squalene epoxidase
About this gene: Sqle enables squalene monooxygenase activity. It is involved in cholesterol biosynthetic process via desmosterol; cholesterol biosynthetic process via lathosterol; and zymosterol biosynthetic process. Is active in cytoplasmic side of endoplasmic reticulum membrane.
Gene details: NCBI Gene ID: 20775; UniProt: P52019; Chromosome location: 15 D1|15 25.16 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.