SYBR Green qPCR primers for detecting mouse Sptlc2 (serine palmitoyltransferase, long chain base subunit 2, also called LCB2a) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Sptlc2 qPCR Primer Set – serine palmitoyltransferase, long chain base subunit 2
Also known as: LCB2, LCB2a, Spt2
About this gene: Sptlc2 encodes a long chain base subunit of serine palmitoyltransferase. The enzyme, serine palmitoyltransferase, consists of two different subunits, and is the key enzyme in sphingolipid biosynthesis. The Sptlc2 gene product catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.
Gene details: NCBI Gene ID: 20773; UniProt: P97363; Chromosome location: 12 D2|12 41.52 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.