SYBR Green qPCR primers for detecting mouse Spdef (SAM pointed domain containing ets transcription factor) gene expression by real-time PCR.
Mouse Spdef qPCR Primer Set – SAM pointed domain containing ets transcription factor
Also known as: PDEF, Pse
About this gene: Spdef is predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity.
Gene details: NCBI Gene ID: 30051; UniProt: Q9WTP3; Chromosome location: 17 A3.3|17 14.59 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.