SYBR Green qPCR primers for detecting mouse Bckdhb (branched chain ketoacid dehydrogenase E1, beta polypeptide, also called BCKDE1B) gene expression by real-time PCR.
Mouse Bckdhb qPCR Primer Set – branched chain ketoacid dehydrogenase E1, beta polypeptide
Also known as: BCKDE1B, BCKDH E1-beta
About this gene: Bckdhb encodes a beta chain of the branched chain alpha ketoacid dehydrogenase complex. The Bckdhb protein exists in a heterotetrameric complex containing the Bckdh alpha subunit to form the E1 catalytic component of Bckdh complex. The Bckdh complex catalyzes the oxidative decarboxylation of branched chain ketoacids to their corresponding acyl-CoA esters, during the catabolism of leucine, isoleucine and valine.
Gene details: NCBI Gene ID: 12040; UniProt: Q6P3A8; Chromosome location: 9 E2|9 45.67 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.