SYBR Green qPCR primers for detecting mouse Bard1 (BRCA1 associated RING domain 1) gene expression by real-time PCR.
Mouse Bard1 qPCR Primer Set – BRCA1 associated RING domain 1
About this gene: Bard1 is predicted to enable RNA binding activity; protein dimerization activity; and ubiquitin protein ligase activity. Predicted to contribute to ubiquitin-protein transferase activity. It is predicted to be involved in multiple processes, including negative regulation of protein export from nucleus; protein K6-linked ubiquitination; and regulation of apoptotic process.
Gene details: NCBI Gene ID: 12021; UniProt: O70445; Chromosome location: 1 C3|1 35.67 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.