SYBR Green qPCR primers for detecting mouse Six3 (sine oculis-related homeobox 3) gene expression by real-time PCR.
Mouse Six3 qPCR Primer Set – sine oculis-related homeobox 3
Also known as: E130112M24Rik, Six3a, Six3alpha, Six3b, Six3beta
About this gene: Six3 enables multiple functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; histone deacetylase binding activity; and transcription corepressor binding activity. It is involved in multiple processes, including camera-type eye development; forebrain development; and lens fiber cell apoptotic process. It acts upstream of or within multiple processes, including forebrain development; lens induction in camera-type eye; and regulation of DNA-templated transcription.
Gene details: NCBI Gene ID: 20473; UniProt: Q62233; Chromosome location: 17 E4|17 55.42 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.