SYBR Green qPCR primers for detecting mouse Sirt5 (sirtuin 5) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Sirt5 qPCR Primer Set – sirtuin 5
Also known as: 0610012J09Rik, 1500032M05Rik
About this gene: Sirt5 enables protein-glutaryllysine deglutarylase activity; protein-malonyllysine demalonylase activity; and protein-succinyllysine desuccinylase activity. It is involved in protein deacylation and regulation of ketone biosynthetic process. It acts upstream of or within peptidyl-lysine desuccinylation and response to ischemia.
Gene details: NCBI Gene ID: 68346; UniProt: Q8K2C6; Chromosome location: 13 A4|13 21.6 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
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