SYBR Green qPCR primers for detecting mouse Rnf13 (ring finger protein 13) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Rnf13 qPCR Primer Set – ring finger protein 13
Also known as: 2010001H16Rik, Rzf
About this gene: Rnf13 belongs to the PA-TM-RING family of proteins that contain a protease associated domain and a RING finger domain separated by a transmembrane domain. The Rnf13 protein is an E3 ubiquitin ligase localized to the endosomal-lysosomal vesicles and inner nuclear membrane. Mice lacking the Rnf13 protein have impaired learning abilities linked to a decreased synaptic vesicle density and dysregulated SNARE complex assembly.
Gene details: NCBI Gene ID: 24017; UniProt: O54965; Chromosome location: 3 D|3 28.04 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
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