SYBR Green qPCR primers for detecting mouse Rap1a (Rap1a member of RAS oncogene family) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Rap1a qPCR Primer Set – Rap1a member of RAS oncogene family
Also known as: G-22K, Krev-1, Rap1
About this gene: Rap1a enables G protein activity and small GTPase binding activity. It is involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway and positive regulation of vasculogenesis. It acts upstream of or within multiple processes, including negative regulation of synaptic vesicle exocytosis; positive regulation of ERK1 and ERK2 cascade; and small GTPase-mediated signal transduction.
Gene details: NCBI Gene ID: 109905; UniProt: P62835; Chromosome location: 3 F2.2|3 46.45 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
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