SYBR Green qPCR primers for detecting mouse Raf1 (Raf1 proto-oncogene, serine/threonine kinase, also called cRaf) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Raf1 qPCR Primer Set – Raf1 proto-oncogene, serine/threonine kinase
Also known as: 6430402F14Rik, Craf1, D830050J10Rik, Raf-1, c-Raf, cRaf, v-Raf
About this gene: Raf1 enables MAP kinase kinase kinase activity and small GTPase binding activity. It is involved in multiple processes, including cell surface receptor protein tyrosine kinase signaling pathway; nervous system development; and neuromuscular junction development. It acts upstream of or within multiple processes, including cell surface receptor signaling pathway; insulin secretion involved in cellular response to glucose stimulus; and negative regulation of extrinsic apoptotic signaling pathway via death domain receptors.
Gene details: NCBI Gene ID: 110157; UniProt: Q99N57; Chromosome location: 6 E3|6 53.62 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.