SYBR Green qPCR primers for detecting mouse Asf1a (anti-silencing function 1A histone chaperone) gene expression by real-time PCR.
Mouse Asf1a qPCR Primer Set – anti-silencing function 1A histone chaperone
Also known as: 2310079C17Rik
About this gene: Asf1a enables chromatin binding activity. It acts upstream of or within muscle cell differentiation and osteoblast differentiation. Predicted to be active in chromatin; nucleus; and site of double-strand break.
Gene details: NCBI Gene ID: 66403; UniProt: Q9CQE6; Chromosome location: 10 B3|10 27.24 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.