SYBR Green qPCR primers for detecting mouse Pitx2 (paired-like homeodomain transcription factor 2, also called Solurshin) gene expression by real-time PCR.
Mouse Pitx2 qPCR Primer Set – paired-like homeodomain transcription factor 2
Also known as: 9430085M16Rik, Brx1, Brx1b, Munc30, Otlx2, Ptx2, Rieg
About this gene: Pitx2 enables DNA-binding transcription factor activity, RNA polymerase II-specific; chromatin DNA binding activity; and sequence-specific DNA binding activity. It is involved in multiple processes, including cardiac neural crest cell migration involved in outflow tract morphogenesis; deltoid tuberosity development; and left/right pattern formation. It acts upstream of or within multiple processes, including circulatory system development; diencephalon development; and digestive tract morphogenesis.
Gene details: NCBI Gene ID: 18741; UniProt: P97474; Chromosome location: 3 G3|3 57.84 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.