SYBR Green qPCR primers for detecting mouse Pgk1 (phosphoglycerate kinase 1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Pgk1 qPCR Primer Set – phosphoglycerate kinase 1
Also known as: Pgk-1
About this gene: The Pgk1 protein is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The Pgk1 protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.
Gene details: NCBI Gene ID: 18655; UniProt: P09411; Chromosome location: X D|X 47.36 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.