SYBR Green qPCR primers for detecting mouse Ndufa7 (NADH:ubiquinone oxidoreductase subunit A7, also called CI-B14.5a) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Ndufa7 qPCR Primer Set – NADH:ubiquinone oxidoreductase subunit A7
Also known as: 14.5kDa, 2400007M02Rik, CI-B14.5a
About this gene: Ndufa7 encodes a subunit of the NADH-ubiquinone oxidoreductase enzyme, which is a large, multimeric protein. The Ndufa7 gene product is the first enzyme complex in the mitochondrial electron transport chain and catalyzes the transfer of electrons from NADH to the electron acceptor ubiquinone. The proton gradient created by electron transfer drives the conversion of ADP to ATP. Complex I has been biochemically separated into four fractions.
Gene details: NCBI Gene ID: 66416; UniProt: Q9Z1P6; Chromosome location: 17 B1|17 17.98 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.