SYBR Green qPCR primers for detecting mouse Mdh1 (malate dehydrogenase 1, NAD (soluble), also called KAR) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Mdh1 qPCR Primer Set – malate dehydrogenase 1, NAD (soluble)
Also known as: B230377B03Rik, KAR, MDH-s, MDHA, Mor-2, Mor2
About this gene: Mdh1 encodes a enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. Mdh1 encodes a cytosolic isozyme, which is central to the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes.
Gene details: NCBI Gene ID: 17449; UniProt: P14152; Chromosome location: 11 A3.1|11 13.89 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.