SYBR Green qPCR primers for detecting mouse Mcm5 (minichromosome maintenance complex component 5, also called P1-CDC46) gene expression by real-time PCR.
Mouse Mcm5 qPCR Primer Set – minichromosome maintenance complex component 5
Also known as: Cdc46, Mcmd5, P1-CDC46
About this gene: The Mcm5 protein belongs to the oligameric minichromosome maintenance protein complex. During DNA replication, the complex loads onto chromatin in early G1 and is converted into an active replicative helicase during S phase. The Mcm5 gene product functions to limit DNA synthesis to once per cell cycle.
Gene details: NCBI Gene ID: 17218; UniProt: P49718; Chromosome location: 8 C1|8 35.59 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.