SYBR Green qPCR primers for detecting mouse Lss (lanosterol synthase, also called OSC) gene expression by real-time PCR.
Mouse Lss qPCR Primer Set – lanosterol synthase
Also known as: 2810025N20Rik, D10Ertd116e, Osc
About this gene: Lss encodes a enzyme that catalyzes the conversion of-2,3 oxidosqualene to lanosterol. The Lss protein belongs to the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D.
Gene details: NCBI Gene ID: 16987; UniProt: Q8BLN5; Chromosome location: 10 C1|10 39.1 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.